Functional analysis of isoflavones using patient-derived human colonic organoids

Inflammatory bowel disease (IBD) comprises two major subtypes, ulcerative colitis (UC) and Crohn’s disease, which are multifactorial diseases that may develop due to genetic susceptibility, dysbiosis, or environmental factors. Environmental triggers of IBD include food-borne factors, a previous nationwide survey in Japan identified pre-illness consumption isoflavones as risk factor for UC. However, the precise mechanisms involved detrimental effects on intestinal mucosa remain unclear. The present study employed human colonic organoids (hCOs) investigate functional effect representative isoflavones, genistein daidzein, epithelial cells. addition organoid reformation assays significantly decreased number size reformed hCOs compared with control daidzein treatment, indicating an inhibitory cell/progenitor cell function. Evaluation phosphorylation status 49 different receptor tyrosine kinases showed selectively inhibited epidermal growth (EGFR) hepatocyte (HGFR). We established two-dimensional wound-repair model using delayed overall response. Our results collectively show exert its via negative regulation stem/progenitor function, possibly leading sustained mucosal injury development